Quantification of selected protein complexes in Synechocystis cells

Range Table - link Molecules per cell
Organism Cyanobacteria Synechocystis PCC 6803
Reference Zavřel T et al., Quantitative insights into the cyanobacterial cell economy. Elife. 2019 Feb 4 8. pii: e42508. DOI: 10.7554/eLife.42508 pp.14-15 table 2PubMed ID30714903
Primary Source Keren N, Aurora R, Pakrasi HB. Critical roles of bacterioferritins in iron storage and proliferation of cyanobacteria. Plant Physiol. 2004 Jul135(3):1666-73 DOI: 10.1104/pp.104.042770 AND Moal G, Lagoutte B. Photo-induced electron transfer from photosystem I to NADP(+): characterization and tentative simulation of the in vivo environment. Biochim Biophys Acta. 2012 Sep1817(9):1635-45. doi: 10.1016/j.bbabio.2012.05.015PubMed ID15247377, 22683536
Method P.14, caption above table 2: "Protein abundances were estimated as molecules per cell, as inferred from mass spectrometry, immunoblotting and spectrophotometric analysis." P.11 2nd paragraph: "Using the combined data of iBAQ [the total intensities divided by the identified peptides for one protein] intensities and quantification by immunoblotting and mass spectrometry, allows [investigators] to provide estimates of absolute amounts of selected protein complexes in Synechocystis cells. The results are summarized in Table 2, details of the calculations are listed in Table 2—Source data 1."
Entered by Uri M
ID 116748