Amount of glycogen per gram dry weight (gDW) with increasing growth rate and under photoinhibition (see 'Comments' section)

Range 84±28 mg/gDW to 199±35 mg/gDW under photoinhibition 229±72 mg/gDW
Organism Cyanobacteria Synechocystis PCC 6803
Reference Zavřel T et al., Quantitative insights into the cyanobacterial cell economy. Elife. 2019 Feb 4 8. pii: e42508. DOI: 10.7554/eLife.42508 p.6 3rd paragraphPubMed ID30714903
Method P.2 bottom paragraph: "The Synechocystis substrain GT-L (Zavřel et al., 2015b) was cultivated in flat panel photobioreactors (Figure 1A) using at least 5 independent reactors in a quasi-continuous (turbidostat) regime (Figure 1B), with red light intensities of 27.5 − 1100 μmol(photons) m^-2s^-1, supplemented with a blue light intensity of 27.5 μmol(photons) m^-2s^-1. The addition of blue light avoids possible growth limitations in the absence of short wavelength photons (Golden, 1995). Steady-state specific growth rates in turbidostat mode were calculated from monitoring the optical density measured at a wavelength of 680 nm (OD680) as well as from the rate of depletion of spare cultivation medium (as measured by top loading balances). Both methods resulted in similar average values (Figure 1C). Estimation of the specific growth rates based on the medium depletion, however, exhibited higher variance. For further analysis, therefore, only values obtained from the OD680 signal are reported."
Comments P.6 3rd paragraph: "The amount of glycogen per gDW increased with increasing growth rate, from 84±28 mg/gDW to 199±35 mg/gDW for the maximal growth rate, and further increased to 229±72 mg/gDW under conditions of photoinhibition (Figure 2G). These values correspond to an increase of glycogen per cell from 440±79 fg/cell to 2329±504 fg/cell (Figure 2H)." Comment from Ron Milo: "Based on reported mass and volume the density seems too high and thus there might be some problem."
Entered by Uri M
ID 116739