Variation (fold) in number of copies of mitochondrial DNA in apparently healthy individuals

Range 2 - 10 fold range
Organism Human Homo sapiens
Reference Shokolenko IN, Alexeyev MF. Mitochondrial DNA: A disposable genome? Biochim Biophys Acta. 2015 Sep1852(9):1805-9. doi: 10.1016/j.bbadis.2015.05.016 p.1807 left column 3rd paragraphPubMed ID26071375
Primary Source [22] Stringer HA, Sohi GK, Maguire JA, Côté HC. Decreased skeletal muscle mitochondrial DNA in patients with statin-induced myopathy. J Neurol Sci. 2013 Feb 15 325(1-2):142-7. doi: 10.1016/j.jns.2012.12.023PubMed ID23312852
Method Primary source [22] abstract: "In this retrospective study, skeletal muscle biopsies from patients diagnosed with SIM [statin-induced myopathy] were studied. These were compared with biopsies from patients clinically assessed as having statin-unrelated myopathy but whose biopsy showed no or negligible pathology. For each biopsy sample, mtDNA was quantified relative to nuclear DNA (mtDNA content) by qPCR, mtDNA deletions were investigated by long-template PCR followed by gel densitometry, and mtDNA oxidative damage was quantified using a qPCR-based assay. For a subset of matched samples, mtDNA heteroplasmy and mutations were investigated by cloning/sequencing."
Comments P.1807 left column 3rd paragraph: "It is often overlooked that normal mtDNA copy number in a given tissue is not a set figure, but is rather a range. Therefore, in most population studies mtDNA copy number in apparently healthy individuals varies over a 2–10 fold range [primary source], and mtDNA content between 40 and 150% of the average is considered clinically normal [ref 23]."
Entered by Uri M
ID 116472