Human nuclear DNA content and cell volume data (to view table please scroll down to pp.7-25)

Range Table - link
Organism Human Homo sapiens
Reference Gillooly JF, Hein A, Damiani R. Nuclear DNA Content Varies with Cell Size across Human Cell Types. Cold Spring Harb Perspect Biol. 2015 Jul 1 7(7):a019091. doi: 10.1101/cshperspect.a019091 table 1 pp.7-25PubMed ID26134319
Primary Source See list of sources on right side of table. Kindly consult full reference list on pp.26-27
Method P.2 left column bottom paragraph: "[Investigators’] analysis for this work used published data from healthy human cell populations representing 19 different cell types, as designated in the original studies (data provided in Table 1). In the original studies, DNA content was estimated using the Feulgen staining method, and the size of cells or cell nuclei were directly measured. Feulgen staining (Feulgen and Rosenbeck 1942) has been the most widely used method for estimating DNA content for several decades, and is still generally considered a reliable method for making quantitative measurements of DNA content (Chieco and Derenzini 1999, Biesterfeld et al. 2011). Measurement errors are typically <5% using this method (Gregory 2005), which for [their] analyses is negligible given the orders of magnitude variation in DNA content among the cells [they] consider. The method works by staining DNA owing to the reaction of Schiff or pseudo-Schiff reagents with aldehydes, which are converted from deoxyribose in DNA after HCl hydrolysis (Chieco and Derenzini 1999). The light absorbance of the stained genetic material is then measured to quantify the relative DNA content of cells."
Comments P.6 right column 2nd paragraph: "However, for the two cell types for which [investigators] have data on the individual cells of a cell population (amnion epithelial cells and hepatocytes), a closer look at the data provides some clues (see Table 1). In these data, the increases in DNA content with cell size appear to cluster around values expected from endopolyploidization (e.g., 7, 14, 28, etc.), but there is considerably more variation than one would expect simply based on error. Thus, the data suggest DNA content has increased as a result of some combination of partial and complete genome duplication in these two cell types." Please see notes beneath table (bottom of p.25)
Entered by Uri M
ID 116372