| Range |
cytoplasm 15: nucleus 60 unitless
|
| Organism |
Chinese hamster ovary (CHO) |
| Reference |
Theillet FX et al., Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs). Chem Rev. 2014 Jul 9 114(13):6661-714. doi: 10.1021/cr400695p p.6664 right column 3rd paragraphPubMed ID24901537
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| Primary Source |
[90] Sasmal DK, Ghosh S, Das AK, Bhattacharyya K. Solvation dynamics of biological water in a single live cell under a confocal microscope. Langmuir. 2013 Feb 19 29(7):2289-98. doi: 10.1021/la3043473PubMed ID23336846
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| Method |
Primary source abstract: "Time-resolved confocal microscopy has been applied to study the cytoplasm and nucleus region of a single live Chinese hamster ovary (CHO) cell. To select the cytoplasm and the nucleus region, two different fluorescent probes are used. A hydrophobic fluorescent dye, DCM, localizes preferentially in the cytoplasm region of a CHO cell. A DNA binding dye, DAPI, is found to be inside the nucleus of the cell. The locations of the probes are clearly seen in the image. Emission maxima of the dyes (DCM in cytoplasm and DAPI in the nucleus) are compared to those of the same dyes in different solvents." |
| Comments |
P.6664 right column 3rd paragraph: "Dielectric constants of 15 and 60 were recently determined for the cytoplasm and cell nucleus of mammalian CHO cells, respectively (primary source)." |
| Entered by |
Uri M |
| ID |
115840 |