Dielectric constants of human colon cancer cells (HT-29 cells)

Range cytoplasm ~50-150: membranes ~2-15 unitless
Organism Human Homo sapiens
Reference Theillet FX et al., Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs). Chem Rev. 2014 Jul 9 114(13):6661-714. doi: 10.1021/cr400695p p.6664 right column 3rd paragraphPubMed ID24901537
Primary Source [88] Wu L, Lanry Yung LY, Lim KM. Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells. Biomicrofluidics. 2012 Mar6(1):14113-1411310. doi: 10.1063/1.3690470PubMed ID22662097
Method Primary source abstract: "In this paper, a new dielectrophoresis (DEP) method based on capture voltage spectrum is proposed for measuring dielectric properties of biological cells. The capture voltage spectrum can be obtained from the balance of dielectrophoretic force and Stokes drag force acting on the cell in a microfluidic device with fluid flow and strip electrodes. The method was demonstrated with the measurement of dielectric properties of human colon cancer cells (HT-29 cells). From the capture voltage spectrum, the real part of Clausius-Mossotti factor of HT-29 cells for different frequencies of applied electric field was obtained. The dielectric properties of cell interior and plasma membrane were then estimated by using single-shell dielectric model."
Comments P.6664 right column 3rd paragraph: "The dielectric properties of many cell types have been investigated, including E. coli (refs 82-84), murine lymphocytes and erythrocytes (ref 85), murine erythroleukemia cells (ref 86), and human breast- and colon-cancer cells (ref 87, primary source), as well as red blood cells (ref 87). It is difficult to draw firm conclusions from these studies because the reported values for the dielectric constants of the cytoplasm range from ∼50–150, while those of membranes range from ∼2–15 (primary source)."
Entered by Uri M
ID 115816