Total cellular concentrations

Range glutamate ∼75: glutathione ∼15: ATP ∼2.5 mM
Organism Budding yeast Saccharomyces cerevisiae
Reference Theillet FX et al., Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs). Chem Rev. 2014 Jul 9 114(13):6661-714. doi: 10.1021/cr400695p p.6664 left column 3rd paragraphPubMed ID24901537
Primary Source [39] van Eunen K et al., Measuring enzyme activities under standardized in vivo-like conditions for systems biology. FEBS J. 2010 Feb277(3):749-60. doi: 10.1111/j.1742-4658.2009.07524.x [67] Østergaard H, Tachibana C, Winther JR. Monitoring disulfide bond formation in the eukaryotic cytosol. J Cell Biol. 2004 Aug 2 166(3):337-45 DOI: 10.1083/jcb.200402120 [68] Ytting CK et al., Measurements of intracellular ATP provide new insight into the regulation of glycolysis in the yeast Saccharomyces cerevisiae. Integr Biol (Camb). 2012 Jan4(1):99-107. doi: 10.1039/c1ib00108fPubMed ID20067525, 15277542, 22134619
Method Primary source [39] abstract: "…[investigators] have developed a single assay medium for determining enzyme-kinetic parameters in yeast." Primary source [67] abstract: "Here, [investigators] present a glutathione-specific green fluorescent protein-based redox probe termed redox sensitive YFP (rxYFP). Using yeast with genetically manipulated GSSG [oxidized glutathione] levels, [they] find that rxYFP equilibrates with the cytosolic glutathione redox buffer." Primary source [68] abstract: "[Investigators] have constructed a new nanobiosensor that can perform time-resolved measurements of intracellular ATP in intact cells."
Comments p.6664 left column 3rd paragraph: "In the yeast S. cerevisiae, total cellular concentrations of glutamate, glutathione, and ATP are ∼75, ∼15, and ∼2.5 mM, respectively (primary sources)."
Entered by Uri M
ID 115810