Length of bacterial cell filaments that can be grazed by mixotrophic nanoflagellate Ochromonas

Range ≤14 µm
Organism Nanoflagellate Ochromonas spp.
Reference Young KD. The selective value of bacterial shape. Microbiol Mol Biol Rev. 2006 Sep70(3):660-703 DOI: 10.1128/MMBR.00001-06 p.685 right column 2nd paragraphPubMed ID16959965
Primary Source [113] Hahn MW, Höfle MG. Flagellate predation on a bacterial model community: interplay of size-selective grazing, specific bacterial cell size, and bacterial community composition. Appl Environ Microbiol. 1999 Nov65(11):4863-72 [259] Posch, T. et al., 1999. Predator-induced changes of bacterial size-structure and productivity studied on an experimental microbial community. Aquat. Microb. Ecol. 18: 235-246 doi:10.3354/ame018235 [375] Wu QL, Boenigk J, Hahn MW. Successful predation of filamentous bacteria by a nanoflagellate challenges current models of flagellate bacterivory. Appl Environ Microbiol. 2004 Jan70(1):332-9PubMed ID10543797, 14711660
Method Primary source [113] abstract: "Before and after the introduction of the predator, the bacterial community composition was studied by in situ techniques (immunofluorescence microscopy and fluorescent in situ hybridization), as well as by cultivation on agar media. The cell sizes of nonspecifically stained and immunofluorescently labeled bacteria were measured by image analysis." Primary source [259] abstract: "The grazing impact of 3 different protozoan species on a mixed bacterial community was studied by means of a simplified and functionally reproducible experimental microbial food web in a 2-stage flow-through system. In the first stage the algae Rhodomonas sp. was grown on an inorganic medium with its accompanying bacterial community (BC) growing on algal exudates. This mixture of algae and bacteria was transferred into 4 second stage vessels: (1) a control, and 3 vessels inoculated with (2) a heterotrophic nanoflagellate, Bodo saltans, (3) a scuticociliate, Cyclidium glaucoma, and (4) a mixotrophic flagellate, Ochromonas sp. Using image analysis techniques [investigators] followed the changes in bacterial size distributions and bacterial to protozoan total biovolume ratios over an experimental period of 15 d. In addition, productivity of the grazed and ungrazed BC was measured using [3H]thymidine and [14C]leucine." Primary source [375] abstract: "High-resolution video microscopy was used to measure contact, capture, and ingestion rates, as well as selectivity of the flagellate feeding."
Comments P.685 right column 2nd paragraph: "Finally, cell filaments up to 14 μm long can be grazed by the mixotrophic nanoflagellate Ochromonas (primary sources), but at only one-half to one-fourth the rate of shorter cells (primary source 375). Bacteria that survive Ochromonas predation are small, filamentous, or stellate chains of up to 900 rod-shaped cells (primary source 113). Once again, the changes are not permanent: the bacterial population returns to its original size distribution when the predator is removed (primary source 113)."
Entered by Uri M
ID 115656