Rate of acid production by organisms resuspended in the presence of various substrates

Range Table - link µequiv. H+/mg protein/min
Organism Bacteria Mycoplasma mycoides var. Capri
Reference Leblanc G, Le Grimellec C. Active K+ transport in Mycoplasma mycoides var. Capri. Net and unidirectional K+ movements. Biochim Biophys Acta. 1979 Jun 13 554(1):156-67 p.163 table IPubMed ID378256
Method P.163 4th paragraph: "Construction and maintenance of the chemical K+ gradient across the membrane rely on glucose utilization by the organisms. Thus, both processes are associated with a high increase in acid production rate of which 30% is accounted for by lactic acid production. It is to be noted that K+-rich cells produce 30% more acid than K+-depleted organisms (Table I)."
Comments P.163 5th paragraph: "In contrast to pyruvate and L-lactate, neither acetate, succinate or 2-ketoglutarate activated K+ transport. Also, no K+ transport was observed after addition of energy-rich compounds such as ATP, acetyl-phosphate or the electron donor system phenazine methosulfate/ascorbate mixture. Finally, it was noted that the increase in acid production correlates with the ability to drive K+ transport across the membrane for each substrate tested (Table I)."
Entered by Uri M
ID 115484