Carbon:cell volume ratios determined from carbon measurements in bacterial cultures or in size-fractionated natural bacterial assemblages

Range (Bjørnsen 1986) 354 mg C/ml: (Lee and Fuhrman 1987) 380 mg C/ml: (Bratbak 1985) 560 mg C/ml
Organism bacteria
Reference Meinhard Simon, Farooq Azam, Protein content and protein synthesis rates of planktonic marine bacteria. Marine ecology progress series. Oldendorf, 1989 Vol 51 pp.201-213 p.210 left column 4th paragraph
Primary Source Bjørnsen, P. K. (1986). Automatic determination of bacterioplankton biomass by image analysis. Appl. environ. Microbiol. 51 1199-1204 AND Lee S, Fuhrman JA. Relationships between Biovolume and Biomass of Naturally Derived Marine Bacterioplankton. Appl Environ Microbiol. 1987 Jun53(6):1298-303 AND Bratbak G. Bacterial biovolume and biomass estimations. Appl Environ Microbiol. 1985 Jun49(6):1488-93PubMed ID16347077, 16347362, 16346817
Method Primary source Bjørnsen abstract: "Image analysis was applied to epifluorescense microscopy of acridine orange-stained plankton samples. A program was developed for discrimination and binary segmentation of digitized video images, taken by an ultrasensitive video camera mounted on the microscope." Primary source Lee and Fuhrman abstract: "In the present study, natural bacterioplankton assemblages were freshly collected, passed through 0.6-mum-pore-size Nuclepore filters to remove larger particulate materials, and diluted for growth in 0.22-mum-pore-size Millipore filter-sterilized unenriched seawater."
Comments P.210 left column 4th paragraph: "[Investigators’] results are consistent with, and explain, recent findings of very high C:cell volume ratios determined from carbon measurements in bacterial cultures or in size-fractionated natural bacterial assemblages: 354 mg C/ml (primary source Bjørnsen 1986), 380 mg C/ml (primary source Lee and Fuhrman, 1987) and 560 mg C/ml (primary source Bratbak 1985)."
Entered by Uri M
ID 115334