Time to construct flagellar hook-basal body structure (HBB)

Range within 30 min
Organism Bacteria Salmonella typhimurium
Reference Singer HM et al., Selective purification of recombinant neuroactive peptides using the flagellar type III secretion system. MBio. 2012 May 29 3(3). pii: e00115-12. doi: 10.1128/mBio.00115-12 p.3 caption to figure 1(a)PubMed ID22647788
Primary Source [39] Karlinsey JE et al., Completion of the hook-basal body complex of the Salmonella typhimurium flagellum is coupled to FlgM secretion and fliC transcription. Mol Microbiol. 2000 Sep37(5):1220-31.PubMed ID10972838
Method Primary source used electron microscopy (EM)
Comments P.3 caption to figure 1(a): "The HBB structure is completed within 30 min (primary source), which coincides with a substrate specificity switch within the flagellar secretion apparatus (indicated by an orange asterisk in the figure) from early to late substrate secretion." Primary source abstract: "At 20 min after induction, the class 2 fliA promoter became active and intracellular FliA protein levels increased, at 30 min after induction, the class 3 fliC promoter was activated."
Entered by Uri M
ID 115223