| Comments |
P.323 right column 4th paragraph: "For log phase GL cells in standard medium (PY) a volume of about 7500µm^3 was found. Other media gave from 17000µm^3 (3.5×PY) to 3000µm^3 in 0.01×PY, a medium capable of supporting log growth with a doubling time of 11.5 h. Cameron (1973) found 12400µm^3 for log phase cells and 6100µm^3 at MCC (maximum cell concentration). James & Read (1957) used projections of pictures of living cells and found 9400µm^3 for T. pyriformis (at 30°C). According to the formula: V= 4/3×pi × (A/2) × (B/2)^2, where A is the major and B is the minor axis, a value of 7500µm^3 corresponds to A = 40 µm, B = 19µm." PY="a medium composed of 0.75 % proteose peptone (PP) + 0.75 % Bacto yeast extract (YE) + 1.5 % glucose + 1 mM-MgSO4 + 50 mMCaCl2 + 100 µM-ferric citrate, pH 6.8." |