Protein concentrations in several nuclear bodies and surrounding nucleoplasm

Range Cajal bodies 136: speckles 162: nucleoli 215: surrounding nucleoplasm 106 mg/mL
Organism African clawed frog Xenopus laevis
Reference Uversky VN. Intrinsically disordered proteins in overcrowded milieu: Membrane-less organelles, phase separation, and intrinsic disorder. Curr Opin Struct Biol. 2017 Jun44: 18-30. doi: 10.1016/ p.24 left column 2nd paragraphPubMed ID27838525
Primary Source [31] K.E. Handwerger, J.A. Cordero, J.G. Gall Cajal bodies, nucleoli, and speckles in the Xenopus oocyte nucleus have a low-density, sponge-like structure Mol Biol Cell, 16 (2005), pp. 202-211 DOI: 10.1091/mbc.E04-08-0742PubMed ID15509651
Method Refraction index-based evaluation of protein concentrations
Comments P.24 left column 2nd paragraph: "Furthermore, it has been shown that the internal density of PMLOs [proteinaceous membrane-less organelles] is only slightly higher than that of the rest of the cytoplasm or nucleoplasm [primary source, ref 32]. In agreement with this increased density, refraction index-based evaluation of protein concentrations in several nuclear bodies found in the Xenopus oocyte nucleus showed that they contain noticeably more proteins (e.g., total protein concentrations were 136, 162, and 215 mg/ml in Cajal bodies, speckles, and nucleoli, respectively) than the surrounding nucleoplasm (106 mg/ml) [primary source]. Since the overall protein concentration inside PMLOs is noticeably higher than the protein content of crowded cytoplasm and nucleoplasm, the interior of these cellular bodies can be described as an overcrowded milieu."
Entered by Uri M
ID 114203