Number of Gag proteins per virion

Range 750 - 5000 Copies/virion
Organism HIV
Reference Dumas F, Haanappel E. Lipids in infectious diseases - The case of AIDS and tuberculosis. Biochim Biophys Acta. 2017 Sep1859(9 Pt B):1636-1647. doi: 10.1016/j.bbamem.2017.05.007 p.1638 caption to figure 1CPubMed ID28535936
Primary Source [220] Briggs JA et al., The stoichiometry of Gag protein in HIV-1. Nat Struct Mol Biol. 2004 Jul11(7):672-5 [221] Chen Y et al., Fluorescence fluctuation spectroscopy on viral-like particles reveals variable gag stoichiometry. Biophys J. 2009 Mar 496(5):1961-9. doi: 10.1016/j.bpj.2008.10.067 [222] W.I. Sundquist, H.-G. Kräusslich HIV-1 assembly, budding, and maturation Cold Spring Harb. Perspect. Med., 2 (2012) link PubMed ID15208690, 19254556, 22762019
Method Primary source [220] abstract: "[Researchers] used cryo-EM and scanning transmission EM..."
Comments p.1638 caption to figure 1C: "C - Budding of HIV virus (adapted from [ref 219]): The Gag protein presents different subdomains involved at different stages of the budding process. The matrix (MA) domain is anchored to the cell membrane via a myristate. This domain also contains basic amino acids that preferentially interact with acidic lipids. The capsid domain (CA) contains amino acids that promote Gag-Gag interactions (oligomerization) that invaginate the membrane and lead to budding. A viral particle contains 750 to 5000 Gag proteins [primary sources]. The nucleocapsid domain (NC) binds to viral RNA that are packaged in the virus particle. Finally, viral proteases cleave Gag to form a mature virus."
Entered by Uri M
ID 114187