Surface density for subconfluent monolayer of Hep G2 cells

Value 300000 cells/cm^2
Organism Human Homo sapiens
Reference Imaizumi Y et al., Proton-sensing transistor systems for detecting ion leakage from plasma membranes under chemical stimuli. Acta Biomater. 2017 Mar 1 50: 502-509. doi: 10.1016/j.actbio.2016.12.018 p.504 right column 4th paragraphPubMed ID27956364
Method P.504 right column 4th paragraph: "HepG2 cells were cultivated on the Ta2O5 gate insulator at subconfluent levels, using a physically adhered adhesive matrix of poly-l-lysine with a thickness of ∼1 nm (as determined using ellipsometry). The system could be used for any types of adhesive cells such as epithelial cells and fibroblasts [ref 22]."
Comments P.504 right column 4th paragraph: "The cells remained securely adhered under the shear stress produced by the flow. With a surface density of 3 × 10^5 cells/cm^2 for the subconfluent monolayer of eukaryotic cells, the rectangular sensing area (10 μm × 340 μm) was occupied by approximately 10 whole cells (Fig. 1b)."
Entered by Uri M
ID 114152