Cell count

Value 1.8e+9 cells/ml Range: ±0.13e+9 cells/ml
Organism Yeast Candida parapsilosis
Reference Krishnan et al. Whole resting cells vs. cell free extracts of Candida parapsilosis ATCC 7330 for the synthesis of gold nanoparticles. AMB Expr (2016) 6 :92 doi: 10.1186/s13568-016-0268-y p.3 left column 3rd paragraphPubMed ID27718213
Method P.3 left column 3rd paragraph: "The amount of gold atoms present in a single Candida parapsilosis cell was determined by equating the cell count to the amount of gold removal by the cells. Number of yeast cells in the 50 g/L cell suspension was determined using haemocytometer. The average number of cells (per mL) was found to be 1.8 (±0.13) × 10^9 cells. To the reaction flask, varying number of cells was added, to which gold (III) chloride was added to the flasks and kept for incubation at 37 °C, 200 rpm for 72 h. The amount of gold chloride added was 3.51 × 10^20 Au atoms. The amount of gold uptake by the cells is determined."
Entered by sarakrish87
ID 113728