| Range |
~100 stable clones
|
| Organism |
Macaque Macaca fascicularis |
| Reference |
Suemori H. Establishment and therapeutic use of human embryonic stem cell lines. Hum Cell. 2006 May19(2):65-70. DOI: 10.1111/j.1749-0774.2006.00011.x p.69 right column 2nd paragraphPubMed ID16879558
|
| Primary Source |
[13] Masataka Furuya, Kentaro Yasuchika, Yasunori Yoshimura, Norio Nakatsuji, Hirofumi Suemori. Electroporation of cynomolgus monkey embryonic stem cells. Genesis 2003 37: 180–7. DOI: 10.1002/gene.10246PubMed ID14666511
|
| Method |
Primary source abstract: "[Investigators] evaluated electroporation as a method to introduce foreign genes into cynomolgus monkey ES cells. [Their] examination has allowed [them] to establish a protocol producing about 100 stably transfected clones from 10^(7) cynomolgus monkey ES cells." |
| Comments |
P.69 right column 2nd paragraph: "For preliminary research, [investigators] optimized electroporation conditions to introduce foreign genes into cynomolgus monkey ES cells [primary source]. Using an SV40-neo expression vector, about 100 stable clones can be consistently produced from 10^7 monkey ES cells after drug selection. Differences in efficiency, however, were observed for other ES cell lines. In addition, [they] compared the transcriptional activities of the PGK-1 [Phosphoglycerate kinase 1], CMV [Cytomegalovirus], and SV40 promoters in cynomolgus monkey ES cells. Although the PGK-1 and SV40 promoters work efficiently in monkey ES cells, the CMV promoter displayed significantly lower activity compared to the others." |
| Entered by |
Uri M |
| ID |
113614 |