60 - 80 %
||Macaque Macaca fascicularis
||Suemori H. Establishment and therapeutic use of human embryonic stem cell lines. Hum Cell. 2006 May19(2):65-70. DOI: 10.1111/j.1749-0774.2006.00011.x p.66 right column top paragraphPubMed ID16879558
|| Suemori H et al., Establishment of embryonic stem cell lines from cynomolgus monkey blastocysts produced by IVF [in vitro fertilization] or ICSI [intracytoplasmic sperm injection]. Dev Dyn. 2001 Oct222(2):273-9. DOI: 10.1002/dvdy.1191PubMed ID11668604
||P.66 left column bottom paragraph: "As reported in previous studies, the methods used to establish human and monkey ES cell lines are essentially the same as those used to generate mouse ES cells. Briefly, inner cell masses isolated from blastocysts are cultivated on a feeder layer of mouse embryonic fibroblasts or other cells. Stem-cell colonies are selected manually from differentiated derivatives. After consecutive selection of
stem cells, undifferentiated ES cells can be maintained stably in culture. These procedures are identical in primates and mice. Under [investigators’] conditions, the efficiency with which [they] can establish ES cell lines from cynomolgus monkey blastocysts is about 60–80% (primary source). This optimized rate is
higher than that typically seen in mice. By using the protocol developed in monkey ES cell establishment, [they]
derived three human ES cell lines from three blastocysts."