Turnover rate of RuBisCO (ribulose-1,5-bisphosphate carboxylase oxygenase)

Range ~3 1/sec
Organism Unspecified
Reference Ma F, Jazmin LJ, Young JD, Allen DK. Isotopically nonstationary 13C flux analysis of changes in Arabidopsis thaliana leaf metabolism due to high light acclimation. Proc Natl Acad Sci U S A. 2014 Nov 25 111(47):16967-72. doi: 10.1073/pnas.1319485111. p.16967 left column bottom paragraphPubMed ID25368168
Primary Source [6] Sage RF (2002) Variation in the k(cat) of Rubisco in C(3) and C(4) plants and some implications for photosynthetic performance at high and low temperature. J Exp Bot 53(369):609–620PubMed ID11886880
Method Abstract: "[Investigators] performed in vivo isotopic labeling of Arabidopsis thaliana rosettes with (13)CO2 and estimated fluxes throughout leaf photosynthetic metabolism by INST-MFA [Isotopically nonstationary metabolic flux analysis]. Plants grown at 200 µmol m^(-2)s^(-1) light were compared with plants acclimated for 9 d at an irradiance of 500 µmol⋅m^(-2)⋅s^(-1)."
Comments P.16967 left column bottom paragraph: "For 95% of all terrestrial plants (i.e., C3 plants), the reductive pentose phosphate (Calvin–Benson–Bassham, or CBB) cycle directly links light and dark reactions and sustains anabolic activities (ref 5). RuBisCO (ribulose-1,5-bisphosphate carboxylase oxygenase) plays a central role in the cycle by carboxylating ribulose-1,5-bisphosphate (RUBP) with CO2 to form two 3-phosphoglycerate (3PGA) molecules. The other 10 enzymes in the CBB cycle regenerate the RUBP substrate to repeat this process. RuBisCO has a low turnover rate (∼3/s, primary source) and also performs a competitive oxygenation side reaction that limits carboxylation activity."
Entered by Uri M
ID 113450