Fraction of cytosines in DNA that are globally methylated

Range ~10.5 % of all cytosines
Organism Thale cress Arabidopsis thaliana
Reference Vidalis A, Živković D, Wardenaar R, Roquis D, Tellier A, Johannes F. Methylome evolution in plants. Genome Biol. 2016 Dec 20 17(1):264. doi: 10.1186/s13059-016-1127-5. p.1 right column 2nd paragraphPubMed ID27998290
Primary Source [59] Zhang X, Yazaki J, Sundaresan A, Cokus S, Chan SWL, Chen H, et al. Genome-wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis. Cell. 2006 126: 1189–201. DOI: 10.1016/j.cell.2006.08.003 [60] Cokus SJ, Feng S, Zhang X, Chen Z, Merriman B, Haudenschild CD, et al. Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning. Nature. 2008 452: 215–9. DOI: 10.1038/nature06745 [61] Lister R, Malley RCO, Tonti-filippini J, Gregory BD, Berry CC, Miller AH, et al. Highly integrated single-base resolution maps of the epigenome in Arabidopsis. Cell. 2008 133: 523–36. DOI: 10.1016/j.cell.2008.03.029 [62] Zilberman D, Gehring M, Tran RK, Ballinger T, Henikoff S. Genome-wide analysis of Arabidopsis thaliana DNA methylation uncovers an interdependence between methylation and transcription. Nat Genet. 2007 39: 61–9. DOI: 10.1038/ng1929PubMed ID16949657, 18278030, 18423832, 17128275
Method Early methylome sequencing studies
Comments P.1 right column 2nd paragraph: "Early methylome sequencing studies of the A. thaliana Columbia reference accession revealed that this model plant methylates about 10.5% of its cytosines globally (30% in context CG, 14% in CHG, and 6% in CHH, approximately), maintains dense methylation within TE [transposable elements] and repeat sequences (at CG, CHG, and CHH sites), and (on average) intermediate methylation levels in gene bodies (mainly at CG sites) [primary sources]." P.1 left column bottom paragraph: "In the model plant Arabidopsis thaliana, cytosine methylation occurs in symmetrical CG and CHG contexts, as well as in asymmetrical CHH sequence contexts (where H = A, T, C) [ref 56]."
Entered by Uri M
ID 113094