Detection limit of real time PCR

Range ≥10 copies of genomic DNA
Organism Generic
Reference Toumazou C et al., Simultaneous DNA amplification and detection using a pH-sensing semiconductor system. Nat Methods. 2013 Jul10(7):641-6. doi: 10.1038/nmeth.2520. p.644 left column 3rd paragraphPubMed ID23749303
Comments P.644 left column 3rd paragraph: "To evaluate the sensitivity and the potential quantitative application of the integrated circuit platform, [investigators] performed on-chip pH-PCR of the human growth hormone 1 (GH1) gene over a tenfold serial dilution of haploid genome per chamber from purified human genomic DNA and compared the performance of the integrated circuit platform with the conventional SYBR green–based quantitative (q)PCR method (Applied Biosystems 'buffered' PCR mixture) using a fluorescence-based thermocycler (Fig. 4a). Both the integrated circuit and the optical platforms resulted in a comparable efficiency of PCR, 91.57% and 93.07%, respectively, and exhibited good linearity, whereby the correlation coefficient was 0.972 for the integrated circuit and 0.990 for the optical platform. Both the integrated circuit and the optical platforms consistently detected as few as 10 copies of genomic DNA (Fig. 4a)."
Entered by Uri M
ID 113080