||Juliane Liepe et al., A large fraction of HLA class I ligands are proteasome-generated spliced peptides, Science 21 October 2016 • Vol 354 Issue 6310 pp.354-359 p.355 middle column 2nd paragraph
|| A. F. Kisselev, T. N. Akopian, K. M. Woo, A. L. Goldberg, The sizes of peptides generated from protein by mammalian 26 and 20 S proteasomes. Implications for understanding the degradative mechanism and antigen presentation. J. Biol. Chem. 274, 3363–3371 (1999). doi:10.1074/jbc.274.6.3363PubMed ID9920878
||P.354 middle column bottom paragraph: "To overcome these problems, [investigators] developed an analytical strategy that accounts for recent discoveries underpinning the PCPS (proteasome-catalyzed peptide splicing) mechanism and can handle the vast proteome-wide human spliced peptide database (fig. S3). With this strategy, [they] initially analyzed the HLA-I–eluted immunopeptidome of the GR lymphoblastoid cell line (GR-LCL) for a deeper coverage of the immunopeptidome, [they] adopted a two-dimensional (2D) peptide prefractionation strategy followed by a hybrid peptide fragmentation method [electron-transfer higher-energy collision dissociation (EThcD)] for peptide identification (refs 3, 4) (fig. S1), supplemented by an adapted target-decoy approach (fig. S4)." HLA= human leukocyte antigen
||P.355 middle column 2nd paragraph: "Spliced peptides were prevalent not only in the HLA-I immunopeptidome but also in the unsorted pool of GR-LCL cell lysate peptides with molecular weight (MW) smaller than 3 kDa, the maximum size of peptides produced in vitro by the proteasome (primary source) (Fig. 1C)."