Effect of microinjected anti-β-COP antibodies on the mobility of ts-O45-G (temperature-sensitive vesicular stomatitis viral membrane glycoprotein)

Range Table - link
Organism Vesicular stomatitis Indiana virus
Reference Storrie B, Pepperkok R, Stelzer EH, Kreis TE. The intracellular mobility of a viral membrane glycoprotein measured by confocal microscope fluorescence recovery after photobleaching. J Cell Sci. 1994 May107 ( Pt 5):1309-19. p.1313 table 1PubMed ID7929637
Method Confocal microscope fluorescence recovery after photobleaching
Comments P.1313 right column 2nd paragraph: "The mobility of ts-O45-G within membranes of the Golgi complex was further determined quantitatively under two different conditions. In the first, the mobility of ts-O45-G accumulated in the TGN (trans-Golgi network) at 20°C was determined and quantified in eight cells as described above (Fig. 7, Table 1). In the second, cells in which ts-O45-G was accumulated for 2.5 hours in the ER (endoplasmic reticulum) were shifted in the presence of cycloheximide for at least 10 minutes to 31°C to allow its transport to the intermediate compartment and the Golgi complex. Injection with rh-P5D4-Fabs was at 39.5°C. Quantification was for 9 cells (Fig.7, Table 1)." See notes beneath table
Entered by Uri M
ID 112940