Intracellular pools of choline and GB (glycine betaine) under conditions of exposure to choline

Range choline >12: GB >45 ng/mg dry weight
Organism Bacteria Pseudomonas aeruginosa
Reference Wargo MJ. Homeostasis and catabolism of choline and glycine betaine: lessons from Pseudomonas aeruginosa. Appl Environ Microbiol. 2013 Apr79(7):2112-20. doi: 10.1128/AEM.03565-12. p.2117 right column 2nd paragraphPubMed ID23354714
Primary Source [65] Fitzsimmons LF, Hampel KJ, Wargo MJ. 2012. Cellular choline and glycine betaine pools impact osmoprotection and phospholipase C production in Pseudomonas aeruginosa. J. Bacteriol. 194: 4718–4726. doi: 10.1128/JB.00596-12.PubMed ID22753069
Method Primary source abstract: "[Investigators] used (13)C nuclear magnetic resonance ((13)C-NMR) to demonstrate that P. aeruginosa maintains both choline and glycine betaine pools under a variety of conditions, in contrast to the transient glycine betaine pool reported for most bacteria. [They] were able to experimentally manipulate the choline and glycine betaine pools by overexpression of the cognate catabolic genes."
Comments P.2117 right column 2nd paragraph: "P. aeruginosa maintains intracellular pools of choline and GB (>12 ng/mg [dry weight] and >45 ng/mg [dry weight], respectively) under conditions of exposure to choline regardless of the osmotic conditions (primary source), in contrast to what happens in the enteric bacteria studied to date."
Entered by Uri M
ID 112938