Influence of the origin of cell monolayer on sperm motion characteristics at 22°C and 37°C

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Organism Human Homo sapiens
Reference Guerin JF , Ouhibi N, Regnier-Vigouroux G, Menezo Y. Movement characteristics and hyperactivation of human sperm on different epithelial cell monolayers. Int J Androl. 1991 Dec14(6):412-22. p.418 table 1 & p.419 table 2PubMed ID1761322
Method Abstract: "Studies of sperm movement characteristics concern mainly sperm swimming between two glass surfaces (as in the Makler chamber). Using automated videomicrography, (CellSoft, Cryo Resources, New York, USA), [investigators] have analysed the movements of human sperm swimming on monolayers of different origins: monkey kidney (Vero) cells, bovine oviduct cells, and human endometrial cells. About 10(5) sperm were deposited upon preparations consisting of monocellular layers adhering to a coverglass, and placed in a deep slide-coverglass system. Experiments were first performed at room temperature then at 37 degrees C."
Comments P.418 2nd paragraph: "Comparison between the cell layers of different origin: At room temperature-The results are listed in Table 1. According to two-factor ANOVA there was no significant difference between the movement characteristics of sperm on the different cell layers of either genital origin (oviduct or endometrial) or non-genital origin (kidney). However, irrespective of the origin of the cell layer, the lateral amplitude of the sperm head displacement was significantly lower than that found in the corresponding conditioned medium. When sperm were moving on a layer of oviduct cells, the curvilinear velocity (VCL) was significantly lower than that observed in the corresponding conditioned medium. There was no significant difference in the percentage of hyperactivation of sperm under the different conditions." P.419 2nd paragraph: "At 37˚C. Incubation of sperm under the different conditions did not affect VCL and ALH (Table 2). In contrast, VSL (P<0.001), LIN [mean linearity](P<0.0l), and HA [‘hyperactivated’](P<0.001) were all increased substantially. VSL and LIN of sperm swimming on either bovine oviduct epithelium or human endometrial cells were significantly lower than VSL and LIN in B2 medium or on Vero cell monolayers respectively. VSL was diminished significantly on oviduct cell monolayer compared to the corresponding conditioned medium. The same comparison revealed no significant difference using the oviduct cell monolayers." VCL=curvilinear velocity. ALH=amplitude of lateral head displacement.
Entered by Uri M
ID 112846