| Comments |
P.16970 left column 5th paragraph: "Despite the increase in photorespiration, the relative flux to sucrose export also increased to support more biomass production in HL-ACC [high light acclimated] plants. Sucrose export flux more than doubled from 11.7 to 26.4 µmol (hexose units)⋅gFW^−1⋅hr^−1, whereas starch production increased marginally from 6.3 to 8.5 µmol (hexose units)⋅gFW^−1⋅hr^−1. Higher carryover starch levels were observed in HL-ACC leaves throughout the diurnal cycle (Fig. S1). HL-ACC plants also had elevated levels of RuBisCO on the basis of leaf area, FW, or chlorophyll and produced thicker leaves with more biomass (Fig. S1). Furthermore, HL-ACC plants produced more seed biomass (i.e., 10 plants produced approximately twice the amount of seeds that resulted in 93% more biomass by weight) with a greater amount of oil, had altered leaf chlorophyll levels (Fig. S1), and had reduced measured concentrations of several of the Calvin cycle intermediates (Table S1)." P.16971 left column bottom paragraph: "This study also illustrates how combined analysis of flux and metabolite profiling data can provide complementary information about cellular reprogramming in response to light. For example, several measured metabolite intermediates appeared to adjust to the long-term high light acclimation through decreased pool sizes (on either a FW or chlorophyll basis) within the CBB [Calvin–Benson–Bassham] cycle (Table S1), even as their interconnected pathway fluxes increased. Although this result was unanticipated, it was consistently obtained in multiple experiments by using sample collection strategies specifically designed to minimize shading or other potential artifacts." LL=Low Light |