Median cell cycle time of embryonic stem [ES] cell

Value 15.8 hours
Organism Human Homo sapiens
Reference Becker KA et al., Self-renewal of human embryonic stem cells is supported by a shortened G1 cell cycle phase. J Cell Physiol. 2006 Dec209(3):883-93. p.885 right column bottom paragraphPubMed ID16972248
Method P.885 right column bottom paragraph: "To complement [investigators'] flow cytometric and microscopic analyses that defined the quantitative distribution of human ES cells in different cell cycle phases, [they] directly measured the duration of the human ES cell cycle. [They] determined the rate of human ES cell proliferation by pulse labeling with CFSE [Carboxy-fluorescein diacetate succinimidyl ester]. CFSE attaches non-specifically to cellular proteins and its stochastic distribution among progeny cells provides a measure of cell division. [They] followed the amount of CFSE autofluorescence per cell by FACS [Fluorescence-Activated Cell Sorting] as a function of time over the course of 3 days. This approach defines the distribution of cells labeled with different fluorescence intensities relative to the total number of cells at the time of labeling (Fig. 3)."
Comments P.885 right column bottom paragraph: "The resulting proliferative index established that human ES cells have a median cell cycle time of 15.8 h (Fig. 3). Because most actively proliferating somatic cells require ∼24–32 h to complete cell division, the CFSE incorporation data clearly demonstrate that human ES cells have an abbreviated cell cycle."
Entered by Uri M
ID 112766