| Value |
1.06e-3
µm^2/sec
|
| Organism |
Human Homo sapiens |
| Reference |
Yan X, Hoek TA, Vale RD, Tanenbaum ME. Dynamics of Translation of Single mRNA Molecules In Vivo. Cell. 2016 May 5 165(4):976-89. doi: 10.1016/j.cell.2016.04.034. p.979 left column 3rd paragraphPubMed ID27153498
|
| Method |
P.987 left column 4th paragraph: "In summary, here [investigators] have developed an imaging method that
enables the measurement of ribosome initiation and translocation rates on single mRNA molecules in live cells." P.987 left column bottom paragraph: "U2OS [human osteosarcoma U2OS cell line] and HEK293 [Human Embryonic Kidney 293 cell] cells were grown in DMEM/5% with Pen/Strep." |
| Comments |
P.979 left column 3rd paragraph: "Because single mRNAs were tethered to the plasma membrane through multiple PP7 [bacteriophage coat protein] molecules and thus through many CAAX membrane insertion domains, the 2D diffusion of mRNAs was extremely slow (1.06 × 10^−3 μm^2/s, n = 211 mRNAs). This slow diffusion made it possible to track individual mRNAs and their associated translation sites for extended periods of time (mean tracking time >1 hr) (Figure S1C)." |
| Entered by |
Uri M |
| ID |
112742 |