12 h control group 5.5±3.0%: 24 h control group 5.6±2.7% %
||Rat Rattus norvegicus
||Gao G et al., Lowering blood ammonia prevents hepatocyte injury and apoptosis. Int J Clin Exp Med. 2015 Aug 158(8):12347-55. p.12350 table 4PubMed ID26550144
||Abstract: "To study hepatocyte injure through establishing the rat model of acute hepatic failure (ALF). ALF rat model was established by administration with D-galactosamine and LPS, and then giving lowering blood ammonia (LBA) treatment. Besides, the intervention groups were injected with ornithine and aspartate. The control groups were injected saline. Blood ammonia, ALT, AST, TNF-α and IL-6 in blood samples were test at 12 hrs and 24 hrs after treatment with LBA. Hepatocyte apoptosis were tested by TUNEL and DNA Ladder. Expression of P53 and SPP1 were detected by RT-PCR."
||P.12352 right column 2nd paragraph: "TUNEL test results: The dark brown apoptotic cells Stained by diaminobenzidine (DAB) and fluorescently labeled apoptosis increased in liver failure group and intervention group compared with the control group. Intervention group decreased (Figures 2 and and3) compared with liver failure group. Except for the control group the apoptosis in 24 h were higher than 12 h (Table 4) (P<0.05). Apoptotic index and ammonia values were positively correlated by Pearson correlation coefficient 0.740 (P<0.01, two-tailed test)."