Comprehensive absolute cellular metabolite concentrations in mammalian iBMK cell, yeast & E. coli

Range Table - link M
Organism Various
Reference Park JO et al., Metabolite concentrations, fluxes and free energies imply efficient enzyme usage. Nat Chem Biol. 2016 May 2. doi: 10.1038/nchembio.2077. Supplementary Information pp.6-7 Supplementary Table 5PubMed ID27159581
Method Abstract: "Here [investigators] use isotope labeling to measure absolute metabolite concentrations and fluxes in Escherichia coli, yeast and a mammalian cell line. [They] then integrate this information to obtain a unified set of concentrations and ΔG for each organism."
Comments P.4 left column bottom paragraph: "Conservation of absolute metabolite concentrations: Given the fundamental connection between ΔG and metabolite concentrations, the similarity of reaction free energies across species suggested similarity in the metabolomes. A compilation of metabolite concentrations, determined by a combination of direct concentration measurements and the above approach for integrating free energy measurements, is provided in Supplementary Table 5." P.5 right column bottom paragraph: "In addition to the methodology for measuring ΔG, a key contribution of this work is a coherent systems-level set of fluxes, free energies and metabolite concentrations for E. coli, yeast and the mammalian cell line iBMK (Figs. 2–5 and Supplementary Tables 1, 4 and 5)." See notes beneath table. iBMK=Immortalized Baby Mouse Kidney Epithelial Cells
Entered by Uri M
ID 112694