Rate of generation of TG[R] (thioguanine-resistant) mutants in normal and malignant lymphocytes

Range Table - link
Organism Human Homo sapiens
Reference Seshadri R, Kutlaca RJ, Trainor K, Matthews C, Morley AA. Mutation rate of normal and malignant human lymphocytes. Cancer Res. 1987 Jan 15 47(2):407-9. p.409 table 2PubMed ID3466691
Primary Source See ref beneath table
Method Abstract: "The genetic stability of normal and neoplastic lymphocytes was compared by using base-line mutation frequency and mutation rate/cell generation. Mutations at the hypoxanthine-guanine phosphoribosyltransferase locus were studied by enumerating thioguanine-resistant cells in a clonogenic assay."
Comments P.407 left column 5th paragraph: "The following established leukemia and lymphoma cell lines were studied in the logarithmic phase of growth: two Burkitt's lymphoma B-cell lines, FMC-HU-1B and HRIK and JM (Jurkat), an acute lymphoblastic leukemia T-cell line. Human lymphocytes were obtained from peripheral blood samples of five healthy volunteer donors." P.408 left column bottom paragraph: "Table 2 shows the results of the experiments to calculate the mutation generation rates. The rates were calculated from the mean mutations observed in 6-9 replicate cultures after expansion for 30-36 and 17-30 days for normal and malignant lymphocytes, respectively. The rate of mutation/cell/generation was 2- to 120-fold greater in malignant cells compared with normal lymphocytes." 6-thioguanine (6-TG) is a purine analogue
Entered by Uri M
ID 112661