| Comments |
P.530 left column bottom paragraph: "These and other observations suggested that the readily releasable pool is depleted after 10–15 s of stimulation at 30 Hz, and that, if stimulation continues beyond 10–15 s, reserve pool vesicles are mobilized and undergo exocytosis. Thus, [investigators] define the RRP as the fraction of the total vesicle pool that is readily available for release with tetanic stimulation from the standpoint of endocytosis, the RRP is defined as the vesicles that can rapidly recycle without a need for endosomal intermediates. [They] show below that a third definition also identifies this population, namely the vesicles that are available for release with low-frequency stimulation. In the present work, [they] have characterized and quantified functional and morphological properties of these pools using optical, electrophysiological, and electron microscopic techniques." |