Fluxes of D-glucose and D-mannitol in red cell ghosts pretreated and measured at higher temperatures

Range Table - link µmoles/cm^3/sec
Organism Human Homo sapiens
Reference Jung CY, Carlson LM, Whaley DA. Glucose transport carrier activities in extensively washed human red cell ghosts. Biochim Biophys Acta. 1971 Aug 13 241(2):613-27. doi:10.1016/0005-2736(71)90059-9 p.625 table IVPubMed ID5159799
Method Abstract:"Human red cell ghosts were prepared and extensively washed in the presence of divalent cations (Ca2+ and Mg2+) and their residual glucose transport carrier activities were studied. 99.18% of the original cellular contents of hemoglobin were removed by the isolation procedures."
Comments P.625 2nd paragraph:"Effect of heat pretreatment on inactivation of D-glucose flux in ghosts: It is a well known fact that enzyme activities or biological phenomena in general show a well defined heat inactivation [ref 26]. In order to study this aspect of the temperature effect on this system, the ghosts were pretreated for 60 min at a high temperature, up to 80°, and the fluxes of glucose and mannitol were measured at these same temperatures (Table IV). The following points are notable. The observed glucose flux at the higher sugar concentration (130.4mM) was increased considerably as the temperature was raised from 37 to 65°. The flux at the lower sugar concentration (5mM), on the other hand, showed a broad optimum at around 45°, with the flux at 65° being less than the maximum by only 30% and still greater than the flux at 23°." See note above table. Red cell ghost="In a blood smear, a large, pale, crescent-shaped cell devoid of internal contents and produced by hemolysis. Such cells are always indicative of disease." [The free dictionary]
Entered by Uri M
ID 112420