Kinetic parameters of D-glucose transport in red cell ghosts at different pH

Range Table - link
Organism Human Homo sapiens
Reference Jung CY, Carlson LM, Whaley DA. Glucose transport carrier activities in extensively washed human red cell ghosts. Biochim Biophys Acta. 1971 Aug 13 241(2):613-27. doi:10.1016/0005-2736(71)90059-9 p.623 table IIIPubMed ID5159799
Method Abstract:"Human red cell ghosts were prepared and extensively washed in the presence of divalent cations (Ca2+ and Mg2+) and their residual glucose transport carrier activities were studied. 99.18% of the original cellular contents of hemoglobin were removed by the isolation procedures."
Comments P.622 2nd paragraph:"The pH effects on D-glucose flux in ghosts: The effects of pH on D-glucose flux in ghosts were also studied. At eight different pH values between 5.2 and 9.0 pH units, the glucose carrier activities were assayed in terms of the rate of D-[14C]glucose exchange flux measured at complete chemical equilibrium across the ghost membranes. Two different concentrations, 5 and 130.4 mM of the sugar were employed. The temperature was fixed at 24°. The flux at each pH tested was found to be fitted as a single first-order process. The results of the experiments are summarized in Fig. 8. The flux at both of the concentrations showed only a small degree of pH dependency. The flux measured at the lower concentration of the permeant only increased less than 15% as the pH was increased from 5.2 to 9.0. The flux measured at the higher concentration of the permeant, however, increased about 30% under the same conditions. The pattern of the change in both cases was roughly linear and no optimum pH could be detected. Sets of values for φmax and Km were estimated at three different pH values and are shown in Table III." See note above table. Red cell ghost="In a blood smear, a large, pale, crescent-shaped cell devoid of internal contents and produced by hemolysis. Such cells are always indicative of disease." [The free dictionary]
Entered by Uri M
ID 112419