Number of RNAP II molecules in nucleus

Value 80200 RNAP II/nucleus Range: ±8,800 RNAP II/nucleus
Organism Mammalian tissue culture cell
Reference Zhao ZW. et al, Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy. Proc Natl Acad Sci U S A. 2014 Jan 14 111(2):681-6. doi: 10.1073/pnas.1318496111. p.684 left column 2nd paragraphPubMed ID24379392
Method Abstract:"Here [investigators] report a reflected light-sheet superresolution microscopy method capable of imaging inside the mammalian nucleus with superior signal-to-background ratio as well as molecular counting with single-copy accuracy. Using reflected light-sheet superresolution microscopy, [they] probed the spatial organization of transcription by RNA polymerase II (RNAP II) molecules and quantified their global extent of clustering inside the mammalian nucleus."
Comments P.684 left column 2nd paragraph:"Moreover, the density of RNAP II molecules observed was found to be 37.2 ± 4.1 molecules per μm^3 (n = 12,482 molecules in eight cells), which translates to a total of 80,200 ± 8,800 RNAP II molecules in the entire nucleus (Materials and Methods), in good agreement with values reported in previous studies (refs 13, 15, 37). Therefore, [investigators] conclude that [they] indeed exhaustively imaged most of the labeled RNAP II molecules in the optical section illuminated."
Entered by Uri M
ID 112321