| Value |
80200
RNAP II/nucleus
Range: ±8,800 RNAP II/nucleus
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Zhao ZW. et al, Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy. Proc Natl Acad Sci U S A. 2014 Jan 14 111(2):681-6. doi: 10.1073/pnas.1318496111. p.684 left column 2nd paragraphPubMed ID24379392
|
| Method |
Abstract:"Here [investigators] report a reflected light-sheet superresolution microscopy method capable of imaging inside the mammalian nucleus with superior signal-to-background ratio as well as molecular counting with single-copy accuracy. Using reflected light-sheet superresolution microscopy, [they] probed the spatial organization of transcription by RNA polymerase II (RNAP II) molecules and quantified their global extent of clustering inside the mammalian nucleus." |
| Comments |
P.684 left column 2nd paragraph:"Moreover, the density of RNAP II molecules observed was found to be 37.2 ± 4.1 molecules per μm^3 (n = 12,482 molecules in eight cells), which translates to a total of 80,200 ± 8,800 RNAP II molecules in the entire nucleus (Materials and Methods), in good agreement with values reported in previous studies (refs 13, 15, 37). Therefore, [investigators] conclude that [they] indeed exhaustively imaged most of the labeled RNAP II molecules in the optical section illuminated." |
| Entered by |
Uri M |
| ID |
112321 |