Lateral diffusion of GFP-tagged membrane proteins in the plasma membrane

Range Table - link µm^2/sec
Organism Bacteria Lactococcus lactis
Reference Mika JT, Schavemaker PE, Krasnikov V, Poolman B. Impact of osmotic stress on protein diffusion in Lactococcus lactis. Mol Microbiol. 2014 Nov94(4):857-70. doi: 10.1111/mmi.12800. Supplementary information table S3PubMed ID25244659
Method FRAP [fluorescent recovery after photobleaching]
Comments P.858 right column bottom paragraph:"For membrane proteins FRAP was performed in the same way as for the cytosolic proteins (Fig. 1B). For the GFP-tagged membrane proteins LacSΔIIA and BcaP, [investigators] determined the following diffusion coefficients: for LacSΔIIA-GFP Dmean = 0.020 μm^2 s^−1 and for BcaP-GFP Dmean = 0.019 μm^2 s^−1 (Fig. 3A, Table S3, n ≥ 22 cells), which is much slower than the Dmedian of GFP or β-galactosidase-GFP in the cytoplasm of L. lactis. These values are similar to those obtained for membrane proteins of similar size expressed in the cytoplasmic membrane of E. coli, 0.017–0.028 μm^2 s^−1 (Kumar et al., 2010)." See notes beneath table
Entered by Uri M
ID 112271