Mean velocity of movement of axoplasmic organelles along purified microtubules

Value 1.42 µm/sec
Organism Squid Loligo pealeii
Reference Vale RD, Schnapp BJ, Reese TS, Sheetz MP. Organelle, bead, and microtubule translocations promoted by soluble factors from the squid giant axon. Cell. 1985 Mar40(3):559-69. p.561 right column bottom paragraphPubMed ID2578887
Method Abstract:"A reconstituted system for examining directed organelle movements along purified microtubules has been developed. Axoplasm from the squid giant axon was separated into soluble supernatant and organelle-enriched fractions... The axoplasmic supernatant also supported movement of microtubules along a glass surface and movement of carboxylated latex beads along microtubules at 0.5 micron/sec."
Comments P.561 right column bottom paragraph:"Organelles moving a sufficient distance for their translocation to be measured reliably had a mean velocity of 1.42µm/sec (n = 8)." Please note-this species is now known as the longfin inshore squid (Doryteuthis pealeii)
Entered by Uri M
ID 112233