Estimated speed of transport in squid giant axons

Range Table - link µm/hour
Organism Squid
Reference Terada S, Kinjo M, Hirokawa N. Oligomeric tubulin in large transporting complex is transported via kinesin in squid giant axons. Cell. 2000 Sep 29 103(1):141-55. p.143 table 1PubMed ID11051554
Method P.141 right column 2nd paragraph:"[Investigators] injected labeled tubulin into squid giant axons and monitored its fluorescent profile by both confocal laser scanning microscopy (CLSM) (Figure 1A) and fluorescence correlation spectroscopy (FCS) (Figure 7A)."
Comments P.143 left column top paragraph:"As shown in Figure 2A and Figure 2B, the fluorescent profile of each protein traveled down the axon anterogradely. Both moving profile showed significant effect due to diffusion, but their transporting speed was much slower than that of axoplasmic vesicles. As shown in Figure 2A and Figure 2B and Table 1, both proteins [tubulin and creatine kinase] were transported at similar speeds and time course at first. The transport speed of tubulin within 20 min after injection was 149.2 ± 43.6 μm/hr (n = 8) and that of creatine kinase was 151.7 ± 49.6 μm/hr (n = 9). But long-term (1 or 2 hr) measurement revealed that the moving speed decreased time-dependently, and the speed of creatine kinase (1 hr, 84.9 ± 11.6 μm/hr [n = 5] 2 hr, 98.2 ± 16.9 μm/hr [n = 5]), was faster than that of tubulin (1 hr, 34.2 ± 14.7 μm/hr [n = 4] 2 hr, 38.7 ± 16.9 μm/hr [n = 4]) (Table 1) the estimated transporting speed of creatine kinase at 1 or 2 hr from injection was about 2.5 times faster than that of tubulin, both of which were compatible within the range of slow axonal transport rate." Animals studied were Heterololigo bleekeri, Todarodes pacificus pacificus, or Loligo pealei.
Entered by Uri M
ID 112176