Diffusion coefficient of PA-GFP-actin [photoactivatable GFP-γ-actin] in CAD cell

Value 3 µm^2/sec
Organism Mouse Mus musculus
Reference Vitriol EA, McMillen LM, Kapustina M, Gomez SM, Vavylonis D, Zheng JQ. Two functionally distinct sources of actin monomers supply the leading edge of lamellipodia. Cell Rep. 2015 Apr 21 11(3):433-45. doi: 10.1016/j.celrep.2015.03.033. p.439 left column 2nd paragraphPubMed ID25865895
Method p.439 left column 2nd paragraph:"[Investigators] used three-dimensional (3D) analysis/modeling with the Virtual Cell platform (see the Supplemental Experimental Procedures for details) to derive the diffusion rate of PA-GFP-actin. For this analysis, [they] used two soluble, non-polymerizable actin mutants (G13R and R62D) to account for movement of G-actin independent from filament assembly and disassembly (Figure S3A)."
Comments p.439 left column 2nd paragraph:"From these data [see 'Method' section above], [investigators] estimated that the diffusion coefficient of PA-GFP-actin was 3 μm^2/s (Figure 5), which falls within the range of previously calculated diffusion coefficients for actin in cells (McGrath et al., 1998)." Please note: Article (probably erroneously) gives units of "3 μm/s2", which should be 3 "μm^2/s"
Entered by Uri M
ID 112140