Estimated G-actin concentration in lamellipodia in B16-F1 mouse melanoma cells

Value 150 μM
Organism Mouse Mus musculus
Reference Kiuchi T, Nagai T, Ohashi K, Mizuno K. Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension. J Cell Biol. 2011 Apr 18 193(2):365-80. doi: 10.1083/jcb.201101035. p.378 left column 2nd paragraphPubMed ID21502360
Primary Source Koestler, S.A., K. Rottner, F. Lai, J. Block, M. Vinzenz, and J.V. Small. 2009. F- and G-actin concentrations in lamellipodia of moving cells. PLoS ONE. 4:e4810. doi:10.1371/journal.pone.0004810PubMed ID19277198
Method FRAP [Fluorescence Recovery After Photobleaching] and electron microscopy experiments
Comments P.378 left column 2nd paragraph: "Recently, G-actin concentration in lamellipodia was estimated as 150 µM by combination of FRAP and electron microscopy experiments (primary source). They concluded that G-actin concentration in lamellipodia is extremely higher than the Cc [critical concentration] of actin filaments."
Entered by Uri M
ID 112138