Length of lamellipodia in N1E-115 cells

Range ~7 μm
Organism Mouse Mus musculus
Reference Kiuchi T, Nagai T, Ohashi K, Mizuno K. Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension. J Cell Biol. 2011 Apr 18 193(2):365-80. doi: 10.1083/jcb.201101035. p.375 left column top paragraphPubMed ID21502360
Primary Source Kiuchi, T., K. Ohashi, S. Kurita, and K. Mizuno. 2007. Cofilin promotes stimulus-induced lamellipodium formation by generating an abundant supply of actin monomers. J. Cell Biol. 177: 465–476. doi:10 .1083/jcb.200610005PubMed ID17470633
Comments P.375 left column top paragraph:"[Investigators] previously showed that the length of lamellipodia was ~7 μm and the rate of actin retrograde flow was 6.9 μm/min in N1E-115 cells (primary source), indicating that the turnover time of F-actin and the rate of F-actin disassembly in lamellipodia are 61 s and 1.6%/s, respectively." Sigma Aldrich link :"The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax)."
Entered by Uri M
ID 112136