Median halflife of protein

Range Saccharomyces cerevisiae 8.8hours Schizosaccharomyces pombe 12.0hours hours
Organism Yeast
Reference Christiano R, Nagaraj N, Fröhlich F, Walther TC. Global proteome turnover analyses of the Yeasts S. cerevisiae and S. pombe. Cell Rep. 2014 Dec 11 9(5):1959-65. doi: 10.1016/j.celrep.2014.10.065. p.1960 left column bottom paragraphPubMed ID25466257
Method p.1959 right column bottom paragraph:"To determine protein turnover rates systematically, [investigators] metabolically labeled the yeasts with heavy isotopes containing lysine (“heavy” lysine, H) and diluted the cells in an excess of normal lysine (“light” lysine, L) at the beginning of the experiment (Figures 1A, S1A, and S1B). [They] analyzed the decay of the heavy lysine signal in the proteome over time by high-resolution mass spectrometry-based proteomics (Schwanhäusser et al., 2011) (Figure 1B)."
Comments P.1960 left column bottom paragraph:"Surprisingly, the vast majority of proteins in both yeasts are very long-lived. The median half-lives in budding and fission yeast in the conditions of [investigators'] experiments are 8.8 and 12.0 hr, respectively (Figure 1D). Remarkably, and in agreement with previous measurements on the turnover of bulk protein mass (Gancedo et al., 1982), distribution of half-lives span two orders of magnitude and range from a few minutes to more than 100 hr in both species (Figure 1D Tables S1 and S2)."
Entered by Uri M
ID 112045