Number of mRNAs per gene per cell

Value 0.4 mRNAs/gene/cell Range: 0.02 - 3 mRNAs/gene/cell
Organism Bacteria Escherichia coli
Reference Moran MA et al., Sizing up metatranscriptomics. ISME J. 2013 Feb7(2):237-43. doi: 10.1038/ismej.2012.94. p.237 left column top paragraphPubMed ID22931831
Primary Source Taniguchi Y, Choi PJ, Li GW, Chen H, Babu M, Hearn J, Emili A, Xie XS. Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science. 2010 Jul 30 329(5991):533-8. Supplementary Table S6PubMed ID20671182
Method High-resolution fluorescence detection of tagged mRNAs
Comments p.237 left column top paragraph:"Classic microbiological studies of the composition of exponentially growing Escherichia coli concluded that each cell harbors ~1380 mRNA molecules (Neidhardt, 1996), a small number compared with other macromolecule inventories (that is, >3000 genes and >2 000 000 proteins). Similarly, recent single-molecule detection in individual E. coli cells based on high-resolution fluorescence detection of tagged mRNAs determined that the number of transcripts per gene per cell averaged only 0.4 (range: 0.02–3 see Supplementary Table S6 in primary source)."
Entered by Uri M
ID 111919