Force generated by RNAP during transcription

Range ≤25 pN
Organism Bacteria Escherichia coli
Reference Malinen AM et al., Active site opening and closure control translocation of multisubunit RNA polymerase. Nucleic Acids Res. 2012 Aug40(15):7442-51. doi: 10.1093/nar/gks383. p.7442 right columnPubMed ID22570421
Primary Source [6] Abbondanzieri EA, Greenleaf WJ, Shaevitz JW, Landick R, Block SM . Direct observation of base-pair stepping by RNA polymerase. Nature 2005 438: 460-465. [8] Wang MD, Schnitzer MJ, Yin H, Landick R, Gelles J, Block SM . Force and velocity measured for single molecules of RNA polymerase. Science 1998 282: 902-907.PubMed ID16284617, 9794753
Comments p.7442 right column:"Despite the apparent lack of direct coupling between catalysis and translocation, Escherichia coli RNAP is a powerful molecular motor that progresses along the DNA at speeds of ~20–90?nt s^-1 and generates a force of up to 25 pN (primary sources)."
Entered by Uri M
ID 111872