| Comments |
p.7 2nd paragraph:"Pausing properties on nucleosomal DNA: Next, [researchers] investigated the transcriptional dynamics of Pol II through the nucleosome by loading a histone octamer on the 601 nucleosome positioning sequence (NPS) (Lowary and Widom, 1998) (Figure 2B,C, Figure 2—figure supplements 2–5). The wild-type enzyme displays a two-fold increase in the apparent pause density upon encountering the nucleosome (Table 2). The mean pause duration on nucleosomal DNA is significantly longer than that on bare DNA (Table 2 Figure 2—figure supplement 1). Similarly, the mutant Pol II displays higher pause density and longer pause duration in the presence of a nucleosome (Table 2)." |