Range: ±1,900 nm^2
||Budding yeast Saccharomyces cerevisiae
||Kukulski W, Schorb M, Kaksonen M, Briggs JAG (2012) Plasma membrane reshaping during endocytosis is revealed by time-resolved electron tomography. Cell 150: 508–520 doi: 10.1016/j.cell.2012.05.046. abstract & p.517 left column bottom paragraphPubMed ID22863005
||Abstract:"[Investigators] directly correlated fluorescence microscopy of key protein pairs with electron tomography. [They] systematically located 211 endocytic intermediates, assigned each to a specific time window in endocytosis, and reconstructed their ultrastructure in 3D. The resulting virtual ultrastructural movie defines the protein-mediated membrane shape changes during endocytosis in budding yeast."
||Abstract:"Scission occurs on average 9 s after initial bending when invaginations are ~100 nm deep, releasing nonspherical vesicles with 6,400 nm^2 mean surface area." p.517 left column bottom paragraph:"The Entire Plasma Membrane Surface Is Internalized by Endocytosis in One Cell Cycle: The surface area of the vesicles was 6,400 nm^2 ± 1,900 nm^2, which represents the mean area of plasma membrane that is being internalized per endocytic event. Based on this number, and considering an average number of 0.43 actin patches per fl of cell volume (Karpova et al., 1998) and an actin patch lifetime of 15 s (Kaksonen et al., 2003), [investigators] estimated that a yeast cell of about 5 µm diameter internalizes its complete plasma membrane surface by endocytosis in about 100 min, corresponding to approximately one round of the budding yeast cell cycle."