Number of proteins that participate in clathrin-mediated endocytosis

Range >60 proteins
Organism Yeast
Reference Berro J, Pollard TD. Local and global analysis of endocytic patch dynamics in fission yeast using a new "temporal superresolution" realignment method. Mol Biol Cell. 2014 Nov 5 25(22):3501-14. doi: 10.1091/mbc.E13-01-0004. p.3501 left columnPubMed ID25143395
Primary Source Kaksonen M, Sun Y, Drubin DG (2003) A pathway for association of receptors, adaptors, and actin during endocytic internalization. Cell 115: 475–487 & Kaksonen M, Toret CP, Drubin DG (2005) A modular design for the clathrin- and actin-mediated endocytosis machinery. Cell 123: 305–320. & Kaksonen M, Toret CP, Drubin DG (2006) Harnessing actin dynamics for clathrin-mediated endocytosis. Nat Rev Mol Cell Biol 7: 404–414. & Mooren OL, Galletta BJ, Cooper JA (2012) Roles for actin assembly in endocytosis. Annu Rev Biochem 81: 661–686. doi: 10.1146/annurev-biochem-060910-094416.PubMed ID14622601, 16239147, 16723976, 22663081
Comments p.3501 left column:"More than 60 proteins participate in clathrin-mediated endocytosis in yeast cells, and actin assembly plays a major role (primary sources). Over ~20 s, actin assembles at the site of endocytosis, forming a “patch” that becomes motile when actin starts to disassemble (primary sources Kaksonen et al., 2003, 2005, 2006 ref Sirotkin et al., 2010 BNID 102266). Although endocytosis in yeast depends on actin, the roles of actin in invagination of clathrin-coated pits, vesicle scission, and vesicle movement are poorly understood."
Entered by Uri M
ID 111864