Experimental results of Balbiani ring diffusion

Range Table - link
Organism Midge Chironomus tentans
Reference Veith R et al., Balbiani ring mRNPs diffuse through and bind to clusters of large intranuclear molecular structures. Biophys J. 2010 Oct 20 99(8):2676-85. doi: 10.1016/j.bpj.2010.08.004. p.2680 table 1PubMed ID20959109
Method p.2677 left column 2nd paragraph:"[Investigators] performed in-depth studies of BR2.1 mRNP and also of tracer molecule mobility by single molecule tracking and fluorescence correlation spectroscopy (FCS) to understand the intranuclear dynamics of mRNPs on different time- and length scales. The complementary techniques of single molecule tracking and FCS and the comparison of tracer mobility in salivary gland cells and mammalian cells revealed new, to [their] knowledge, aspects of nucleoplasmic mRNP dynamics and provided a rationale for the occurrence of the different BR mRNP mobility components."
Comments p.2680 left column 2nd paragraph:"To view the molecular dynamics from a different perspective, [investigators] calculated the MSDs [mean-square displacements] for all trajectories with an equal weight for each trajectory (see Materials and Methods). This approach guaranteed that retarded and therefore very long trajectories were not overweighted, which would yield a misleadingly low diffusion coefficient. Despite this approach, a clear deviation from linear behavior indicating anomalous diffusion was found (Fig. 2 B). The results of the jump distance and the MSD analysis were summarized in Tables 1 and 2." See notes beneath table
Entered by Uri M
ID 111855