Properties of mitochondria and their genomes

Range Table - link
Organism Human Homo sapiens
Reference Iborra FJ, Kimura H, Cook PR. The functional organization of mitochondrial genomes in human cells. BMC Biol. 2004 May 24 2: 9. p.4 table 1PubMed ID15157274
Primary Source [14] Jacobs HT, Lehtinen SK, Spelbrink JN: No sex please, we're mito- chondria: a hypothesis on the somatic unit of inheritance of mammalian mtDNA. Bioessays 2000, 22: 564-572. [26] Kellenberger E: Intracellular or ganization of the bacterial genome. In The bacterial chromosome Edited by: Drlica K, Riley M. Washington: American Society for Microbiology 1990:173-186.PubMed ID10842310
Method p.3 right column 2nd paragraph:"The number of mitochondrial genomes per focus was calculated after measuring the number of mitochondrial genomes per cell, the total mitochondrial length, and the number of foci per unit length of mitochondria (Table 1). While the first two can be estimated reasonably accurately, it is difficult to be sure that all foci are detected. Therefore [investigators] used various approaches, reasoning that if different methods with different thresholds of detection gave similar results, one estimate would lend credibility to another (Table 1, line 6). The data were analyzed as follows: (i) immunolabelling with both anti-DNA and anti-BrdU (after growth in BrdU to substitute DNA fully) gave similar results (that is, ~7 genomes/focus) (ii) the average intensity of foci in images like that in Figure 1D had an intensity 9.2× higher than the weakest (Figure1F, legend) if the faintest focus then contains one genome, the average would contain ~9 (iii) foci had a diameter of ~68 nm (Table 1, line 4), sufficient to accommodate ~6 genomes packed as tightly as in the bacterial nucleoid [primary source 26] and (iv) analysis of replication rates are consistent with each focus containing ~10 genomes (below). Taken together, these results suggest the average focus contains 6–10 genomes, again consistent with earlier results [refs 18,19]."
Comments A stable human cell line (ECV304, which is also known as T-24). See notes beneath table
Entered by Uri M
ID 111751