| Range |
25 - 30 min
|
| Organism |
Bacteriophage T4 |
| Reference |
Vafabakhsh R et al., Single-molecule packaging initiation in real time by a viral DNA packaging machine from bacteriophage T4. Proc Natl Acad Sci U S A. 2014 Oct 21 111(42):15096-101. doi: 10.1073/pnas.1407235111. p.15100 right column 4th paragraphPubMed ID25288726
|
| Method |
p.15097 left column 3rd paragraph:"[Investigators] used prism-type total internal reflection microscopy (ref 23) to probe individual phage T4 DNA packaging machines. Packaging complexes were first assembled in solution by incubating the purified capsid particles with the motor protein gp17 in the presence of ATP?S and a 120-bp “priming” DNA (unlabeled, discussed below) (Fuller et al. 2007 PMID 17942694)." |
| Comments |
p.15100 right column 4th paragraph:"[Investigators’] real-time single-molecule assay allowed the dissection of some of the steps involved in packaging initiation. When individual packaging machines were imaged each machine showed repeated initiation and translocation of DNA molecules into the virus capsid one after another. [Their] kinetic measurements showed that it takes about 45 s to form an active initiation complex de novo and about 10 s for a preassembled motor to capture a free dsDNA end and initiate translocation. Because the time needed for the elongation (translocation) phase of ~170-kb T4 genome is about 7 min (Fuller et al. 2007 PMID 17942694), the ~1 min of time it takes to initiate packaging de novo is consistent with the ~10 min of time available to complete packaging in the 25- to 30-min infection cycle of phage T4." |
| Entered by |
Uri M |
| ID |
111744 |